DNA contamination in Pfizer and Moderna mRNA vaccines

Resume:

• Pfizer and Moderna mRNA vaccines contain much more DNA contamination than allowed
• This DNA contamination in the mRNA vaccines contains the DNA that codes for the complete spike protein.
• These findings are easily replicated by molecular biology laboratories
• Research into this by the RIVM and/or European Medicines Agency (EMA) is indicated
• Presence of spike-coding DNA in the vaccine could lead to unwanted integration into human DNA
• Long-lasting post-vaccination effects in patients such as chronic inflammatory reactions of the heart, blood vessels, skin or nervous system associated with long-term spike expression could be partly explained by this
• People with post-vaccination complaints could easily be tested for undesired expression of the spike protein and spike gene integration into their DNA using immunohistological and molecular detection techniques.

DNA contamination

The DNA encoding the spike protein is used to produce the LNP mRNA vaccine against Covid-19. The process is called in vitro-transcription, which uses DNA as a matrix to produce mRNA. The spike gene is located on a so-called plasmid-DNA. In addition to the spike gene, this plasmid also contains resistance genes for the antibiotics kanamycin-neomycin and/or ampicillin. Also, the plasmids sometimes contain an element that allows multiplication in human cells, the so-called SV40 origin of replication. The DNA gene encoding spike is preceded by the T7 promoter, an element that allows high mRNA expression in bacteria, but only if they are infected by a bacterial virus, the so-called T7 bacteriophage. The T7 promoter is normally low active in human cells. It now appears that the corona vaccines contain not only pure mRNA, but also (parts of) the plasmid DNA used for the production of the mRNA vaccines from Pfizer and Moderna (1). That is a potential danger because of the potential for long-term production of the toxic spike protein by cells in various organ systems.

By injecting lipid-enclosed DNA complexes, such as are undesirably present in the Pfizer and Moderna vials, the DNA can be taken up by the human cell and then possibly incorporated into the DNA. This allows the spike DNA gene to start producing new spike mRNA. This will happen if the spike DNA is accidentally incorporated into an active region of the human genome. The mRNA technology has always been considered genetically safe because mRNA cannot simply be incorporated into the human genome, something that DNA can do.

According to several published studies (2-7), the spike protein can be found in the human body for many months after vaccination, for example in skin, heart, brain and lymph nodes. It is so far unclear how this is possible. The assumption was that the vaccine mRNA and/or the spike protein is very stable. However, the discovery of spike gene DNA in the mRNA vaccines has raised the possibility that spike mRNA can be newly produced by cells that have incorporated this plasmid DNA.

According to the specifications of it European Medicines Agency (EMA) some DNA contamination is allowed in the mRNA vaccines, up to a maximum of 0.033% (8). However, the amounts found are 20 to 1000 times higher. In addition, it was not known until now that the contamination would concern the intact spike gene. Other potentially harmful genetic elements have also been found, such as genes that can cause resistance to antibiotics.

Further research into this is urgently required, because a vaccine contaminated with spike plasmid DNA may partly explain long-term post-vaccination symptoms, for which adequate diagnoses and therapies are currently lacking. A diagnostic approach could be a combination of anti-spike immunohistochemistry (2), PCR and Sanger sequencing, and/or in situ hybridization specific for vaccine or viral spike mRNA and/or DNA.

The current research was done by a team around genome expert Dr. Kevin McKernan, formerly leader of Research & Development for the Human Genome Project at the Massachusetts Institute of technology (MIT); founder and CSO (Chief Scientific Officer, head of a scientific research team) of the Cannabis Genomes company Medicinal Genomics. The underlying data and protocols are complete open-source and easy to replicate for molecular biology laboratories.

Don’t wait

It can be argued against the current study that (1) the article has not yet been published peer-reviewed is, (2) it concerns a limited number of vials that were also past the expiration date and (3) that the principal investigator works in a company that deals with cannabis genomes, among other things.

Why we bring it to the attention is because (1) the article describes clear and verifiable research, from a molecular biological and genomic perspective, (2) the research based on the described methodology can easily be replicated by specialized laboratories, (3 ) the DNA contamination cannot be explained by the aging of the vials and (4) the principal investigator is demonstrably an expert on genomes.

The Doctors Collective therefore sees these data as grounds for a moratorium on mRNA vaccines until they have been tested for safety, including reproductive safety, in both humans and animals in the usual manner for genetic interventions, and calls for parliamentary questions to be put to the Minister of VWS about this contamination.

Agilent Genomic DNA Screen Tape of an undiluted vaccine preparation. 300 µl of vaccine was CTAB purified and eluted in 300 µl of MGC Elution Buffer (Tris based) to maintain the original vaccine volumes. 7.5-11.3 ng/ul is reported. The blue line on the right (9347) shows the linear vector DNA containing the complete spike gene.

References:

1. McKernan, K., Helbert, Y., Kane, LT, & McLaughlin, S. (2023, April 10). Sequencing of bivalent Moderna and Pfizer mRNA vaccines reveals nanogram to microgram quantities of expression vector dsDNA per dose. https://doi.org/10.31219/osf.io/b9t7m
2. Mörz M. A Case Report: Multifocal Necrotizing Encephalitis and Myocarditis after BNT162b2 mRNA Vaccination against COVID-19. Vaccines (Basel). 2022 Oct 1;10(10):1651. doi: 10.3390/vaccines10101651. PMID: 36298516; PMCID: PMC9611676.
3. Yamamoto M., Kase M., Sano H., Kamijima R., Sano S. Persistent varicella zoster virus infection following mRNA COVID-19 vaccination was associated with the presence of encoded spike protein in the lesion. J Cutan. Immunol. allergy. 2022 doi: 10.1002/cia2.12278.
4. Magen, E.; Mukherjee, S.; Bhattacharya, M.; Detroja, R.; Merzon, E.; Blum, I.; Livoff, A.; Shlapobersky, M.; Baum, G.; Talisman, R.; et al. Clinical and Molecular Characterization of a Rare Case of BNT162b2 mRNA COVID-19 Vaccine-Associated Myositis. Vaccines 2022, 10, 1135.
5. Roltgen, K.; Nielsen, S.C.A.; Silva, O.; Younes, S.F.; Zaslavsky, M.; Costales, C.; Yang, F.; Wirz, OR; Solis, D.; Hoh, R. A.; et al. Immune imprinting, breadth of variant recognition, and germinal center response in human SARS-CoV-2 infection and vaccination. Cell 2022, 185, 1025–1040.e14.
6. Bansal, S.; Perincheri, S.; Fleming, T.; Poulson, C.; Tiffany, B.; Bremner, R.M.; Mohanakumar, T. Cutting Edge: Circulating Exosomes with COVID Spike Protein Are Induced by BNT162b2 (Pfizer-BioNTech) Vaccination prior to Development of Antibodies: A Novel Mechanism for Immune Activation by mRNA Vaccines. J. Immunol. Baltim. Md. 1950 2021, 207, 2405–2410.
7. Baumeier, C.; Aleshcheva, G.; Harms, D.; Gross, U.; Hamm, C.; Assmus, B.; Westenfeld, R.; Kelm, M.; Rammos, S.; Wenzel, P.; et al. Intramyocardial Inflammation after COVID-19 Vaccination: An Endomyocardial Biopsy-Proven Case Series. Int. J Mol. Sci. 2022, 23, 6940.
8. EMA documentation on 330ng/mg (0.033%) DNA/RNA limit. Page 74

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